Glucagon - RIE

Descrição: Glucagon RIA

Informação: Glucagon é um hormônio de 3,5 kDa secretado pelas células A  das ilhotas de Langerhans no pâncreas, e esta envolvido no controle da homeostase de glicose, mobilização de glicose no fígado e secreção de insulina estimulada pela glicose.

Aplicações: 
Dois dias de ensaio de desiquilíbrio a 4°C. Volume de amostra < 100 µL Plasma, soro ou meio de cultura de tecido.

Reatividade: Mamíferos

Precisão, %:

  • Inter-ensaio: 11.7 ± 3.0
  • Intra-ensaio: 4.9 ± 1.3

 

Áreas terapêuticas:

    • Diabetes
    • Obesidade
    • Desordem Metabólica

 

 

Intervalo da curva:  20–400 pg/mL

 

Exatidão:  97 ± 0.8%

 

Reatividade Cruzada:
Glucagon é conservado em quase todos os mamíferos. Este kit pode ser utilizado para a dosagem de glucagon nestas amostras (ex. rato, camundongo, etc.)

Desempenho: 

  • ED80 = 44 ± 4 pg/mL
  • ED50 = 109 ± 5 pg/mL
  • ED20 = 264 ± 17 pg/mL

 

Proteína:  Glucagon

DOCUMENTO

Protocolo

REFERÊNCIA DE PUBLICAÇÕES PARA ESTE KIT

  • Chronic social defeat stress disrupts regulation of lipid synthesis.
    Chuang JC, Cui H, Mason BL, Mahgoub M, Bookout AL, Yu HG, Perello M, Elmquist JK, Repa JJ, Zigman JM, Lutter M (2010) Journal of lipid research
  • Insulinostatic activity of cerebellin--evidence from in vivo and in vitro studies in rats.
    Strowski MZ, Kaczmarek P, Mergler S, Wiedenmann B, Domin D, Szwajkowski P, Wojciechowicz T, Skrzypski M, Szczepankiewicz D, Szkudelski T, Rucinski M, Malendowicz LK, Nowak KW (2009) Regulatory peptides.157:19-24
  • Performance and metabolic and endocrine changes with emphasis on glucose metabolism in high-yielding dairy cows with high and low fat content in liver after calving.
    H M Hammon, G Stürmer, F Schneider, A Tuchscherer, H Blum, T Engelhard, A Genzel, R Staufenbiel, W Kanitz (2009) Journal of dairy science.92:1554-66
  • Impact of Sur1 gene inactivation on the morphology of mouse pancreatic endocrine tissue.
    Ihsane Marhfour, Pierre Moulin, Joëlle Marchandise, Jacques Rahier, Christine Sempoux, Yves Guiot (2009) Cell and tissue research.335:505-15
  • Acute metabolic responses of postpartal dairy cows to subcutaneous glucagon injections, oral glycerol, or both.
    M A Osman, P S Allen, N A Mehyar, G Bobe, J F Coetzee, K J Koehler, D C Beitz (2008) Journal of dairy science.91:3311-22
  • Inhibition of Ca2+ signaling and glucagon secretion in mouse pancreatic alpha-cells by extracellular ATP and purinergic receptors.
    Eva Tudurí, Eliane Filiputti, Everardo M Carneiro, Ivan Quesada (2008) American journal of physiology. Endocrinology and metabolism.294:E952-60
  • Lupin protein influences the expression of hepatic genes involved in fatty acid synthesis and triacylglycerol hydrolysis of adult rats.
    Anja Bettzieche, Corinna Brandsch, Kristin Weisse, Frank Hirche, Klaus Eder, Gabriele I Stangl (2008) The British journal of nutrition.99:952-62
  • Expression of proglucagon and proglucagon-derived peptide hormone receptor genes in the chicken.
    Mark P Richards, John P McMurtry (2008) General and comparative endocrinology.156:323-38
  • cAMP-dependent signaling regulates the adipogenic effect of n-6 polyunsaturated fatty acids.
    Lise Madsen, Lone Møller Pedersen, Bjørn Liaset, Tao Ma, Rasmus Koefoed Petersen, Sjoerd van den Berg, Jie Pan, Karin Müller-Decker, Erik D Dülsner, Robert Kleemann, Teake Kooistra, Stein Ove Døskeland, Karsten Kristiansen (2008) The Journal of biological chemistry.283:7196-205
  • Synergistic impairment of glucose homeostasis in ob/ob mice lacking functional serotonin 2C receptors.
    Jennifer M Wade, Punita Juneja, Adrienne W MacKay, James Graham, Peter J Havel, Laurence H Tecott, Evan H Goulding (2008) Endocrinology.149:955-61
  • Metabolic changes and net portal flux in dairy cows fed a ration containing rumen-protected fat as compared to a control diet.
    H M Hammon, C C Metges, P Junghans, F Becker, O Bellmann, F Schneider, G Nürnberg, P Dubreuil, H Lapierre (2008) Journal of dairy science.91:208-17
  • Gender-associated differences in metabolic syndrome-related parameters in Göttingen minipigs.
    Berit Oestergaard Christoffersen, Nanna Grand, Valeria Golozoubova, Ove Svendsen, Kirsten Raun (2007) Comparative medicine.57:493-504
  • A high-fat, ketogenic diet induces a unique metabolic state in mice.
    Adam R Kennedy, Pavlos Pissios, Hasan Otu, Russell Roberson, Bingzhong Xue, Kenji Asakura, Noburu Furukawa, Frank E Marino, Fen-Fen Liu, Barbara B Kahn, Towia A Libermann, Eleftheria Maratos-Flier (2007) American journal of physiology. Endocrinology and metabolism.292:E1724-39
  • Phlorizin induces lipolysis and alters meal patterns in both early- and late-lactation dairy cows.
    B J Bradford, M S Allen (2007) Journal of dairy science.90:1810-5
  • The effects of combined versus selective adrenergic blockade on left ventricular and systemic hemodynamics, myocardial substrate preference, and regional perfusion in conscious dogs with dilated cardiomyopathy.
    Lazaros A Nikolaidis, Indu Poornima, Pratik Parikh, Megan Magovern, You-Tang Shen, Richard P Shannon (2006) Journal of the American College of Cardiology.47:1871-81
  • The effect of production level on feed intake, milk yield, and endocrine responses to two fatty acid supplements in lactating cows.
    K J Harvatine, M S Allen (2005) Journal of dairy science.88:4018-27
  • Hormonal and metabolic defects in a prader-willi syndrome mouse model with neonatal failure to thrive.
    M Stefan, H Ji, R A Simmons, D E Cummings, R S Ahima, M I Friedman, R D Nicholls (2005) Endocrinology.146:4377-85
  • Glucose production and substrate cycle activity in a fasting adapted animal, the northern elephant seal.
    Cory D Champagne, Dorian S Houser, Daniel E Crocker (2005) The Journal of experimental biology.208:859-68
  • Recurrent insulin-induced hypoglycemia causes site-specific patterns of habituation or amplification of CNS neuronal genomic activation.
    S A Paranjape, K P Briski (2005) Neuroscience.130:957-70
  • Increased insulin sensitivity in paternal Gnas knockout mice is associated with increased lipid clearance.
    Min Chen, Martin Haluzik, Nicole J Wolf, Javier Lorenzo, Kelly R Dietz, Marc L Reitman, Lee S Weinstein (2004) Endocrinology.145:4094-102
  • Functional, persistent, and extended liver to pancreas transdifferentiation.
    Idit Ber, Keren Shternhall, Shira Perl, Zohar Ohanuna, Iris Goldberg, Iris Barshack, Luna Benvenisti-Zarum, Irit Meivar-Levy, Sarah Ferber (2003) The Journal of biological chemistry.278:31950-7
  • The GLP-1 derivative NN2211 restores beta-cell sensitivity to glucose in type 2 diabetic patients after a single dose.
    Annette M Chang, Grethe Jakobsen, Jeppe Sturis, Marla J Smith, Cathie J Bloem, Bob An, Andrzej Galecki, Jeffrey B Halter (2003) Diabetes.52:1786-91
  • Hepatic expression of a targeting subunit of protein phosphatase-1 in streptozotocin-diabetic rats reverses hyperglycemia and hyperphagia despite depressed glucokinase expression.
    Ruojing Yang, Christopher B Newgard (2003) The Journal of biological chemistry.278:23418-25
  • Lipolytic and metabolic response to glucagon in fasting king penguins: phase II vs. phase III.
    Servane F Bernard, Marie-Anne Thil, Rene Groscolas (2003) American journal of physiology. Regulatory, integrative and comparative physiology.284:R444-54
  • Hypoglycemia and reduced feed intake in broiler chickens treated with metformin.
    C M Ashwell, J P McMurtry (2003) Poultry science.82:106-10
  • Bedtime administration of NN2211, a long-acting GLP-1 derivative, substantially reduces fasting and postprandial glycemia in type 2 diabetes.
    Claus B Juhl, Malene Hollingdal, Jeppe Sturis, Grethe Jakobsen, Henrik Agersø, Johannes Veldhuis, Niels Pørksen, Ole Schmitz (2002) Diabetes.51:424-9
  • Over-expression of FoxM1 stimulates cyclin B1 expression.
    Leung TW, Lin SS, Tsang AC, Tong CS, Ching JC, Leung WY, Gimlich R, Wong GG, Yao KM (2001) FEBS Lett.507:59-66.
  • Phentolamine inhibits exocytosis of glucagon by Gi2 protein-dependent activation of calcineurin in rat pancreatic alpha -cells.
    M Høy, K Bokvist, W Xiao-Gang, J Hansen, K Juhl, P O Berggren, K Buschard, J Gromada (2001) The Journal of biological chemistry.276:924-30
  • Regulation of glucagon release in mouse -cells by KATP channels and inactivation of TTX-sensitive Na+ channels.
    S O Göpel, T Kanno, S Barg, X G Weng, J Gromada, P Rorsman (2000) The Journal of physiology.528:509-20
  • Indirect effect of insulin to suppress endogenous glucose production is dominant, even with hyperglucagonemia.
    S D Mittelman, Y Y Fu, K Rebrin, G Steil, R N Bergman (1997) The Journal of clinical investigation.100:3121-30

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    Somente para pesquisa, não é destinado ao uso terapêutico ou diagnóstico.

    Código do ProdutoGL-32K
    Tipo de Produto RIE Obesidade e Diabetes Glucagon
    Fornecedor MILLIPORE
    Quantidade250 tubos
    EspécieNão-específico
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