Adiponectina Humana RIE

Human Adiponectin RIA

Adiponectina é identificada como uma adipocina de 30 kDa que é secretada no tecido adiposo e influência o metabolismo sistêmico.

Aplicações: Radioimunoensaio

Notas:
Incubação overnight a temperatura ambiente.
Volume de amostra - <2 µL de soro ou plasma, ou <100 µL de meio de cultura de tecido

Reatividade: Humano

Precisão, %:

  • Inter-ensaio: 6.9 – 9.3
  • Intra-ensaio: 1.8 – 6.2

     

    Áreas terapêuticas:
  • Diabetes
  • Obesidade
  • Desordem Metabólica

 

Sensibilidade: 0.78 ng/mL

Exatidão:

  • 116% (serum sample concentration 1:500 dilution–5 ng/mL standard)
  • 111% (serum sample concentration 1:500 dilution–20 ng/mL standard)
  • 101% (serum sample concentration 1:500 dilution–100 ng/mL standard)

 

Intervalo da curva: 0.78–200 ng/mL

Especificidade:

Adiponectina humana - 100%
C1q Humana < 0.01%~
Adiponectina de camundongo- 400%

 

Linearidade de Diluição: 85–119%

Desempenho:
ED80 = 3.9 ± 0.9 ng/mL
ED50 = 21.0 ± 5.7 ng/mL
ED20 = 128.0 ± 48.6 ng/mL

Proteína: Adiponectina

DOCUMENTO

Protocolo

REFERÊNCIA DE PUBLICAÇÕES PARA ESTE KIT

  • Relationships between contraction properties of knee extensor muscles and fasting IGF-1 and adipocytokines in physically active postmenopausal women.
    Jürimäe T, Pääsuke M, Kums T, Gapeyeva H, Ereline J, Saar M, Jürimäe J (2010) Clin Physiol Funct Imaging
  • Postprandial plasma adiponectin response is reduced in prepubertal premature pubarche girls.
    Larque E, Gil-Campos M, Villada I, Ramirez-Tortosa M, Canete R, Gil A (2010) Metabolism: clinical and experimental
  • Menopause impacts the relation of plasma adiponectin levels with the metabolic syndrome.
    Henneman P, Janssens AC, Zillikens MC, Frolich M, Frants RR, Oostra BA, van Duijn CM, van Dijk KW (2009) Journal of internal medicine
  • Relationship Between Cerebrospinal Fluid Visfatin (PBEF/Nampt) Levels and Adiposity in Humans.
    Manfred Hallschmid, Harpal Randeva, Bee K Tan, Werner Kern, Hendrik Lehnert (2009) Diabetes.58:637-40
  • Metabolic syndrome affects fatty acid composition of plasma lipids in obese prepubertal children.
    Mercedes Gil-Campos, Maria del Carmen Ramírez-Tortosa, Elvira Larqué, Javier Linde, Concepción M Aguilera, Ramón Cañete, Angel Gil (2008) Lipids.43:723-32
  • Dietary sugars stimulate fatty acid synthesis in adults.
    Elizabeth J Parks, Lauren E Skokan, Maureen T Timlin, Carlus S Dingfelder (2008) The Journal of nutrition.138:1039-46
  • The influence of ghrelin, adiponectin, and leptin on bone mineral density in healthy postmenopausal women.
    Jaak Jürimäe, Toivo Jürimäe, Aire Leppik, Tatjana Kums (2008) Journal of bone and mineral metabolism.26:618-23
  • Plasma levels of interleukin-6 and C-reactive protein are associated with physical inactivity independent of obesity.
    C P Fischer, A Berntsen, L B Perstrup, P Eskildsen, B K Pedersen (2007) Scandinavian journal of medicine science in sports.17:580-7
  • GHR exon 3 polymorphism: association with type 2 diabetes mellitus and metabolic disorder.
    R J Strawbridge, L Kärvestedt, C Li, S Efendic, C G Ostenson, H F Gu, K Brismar (2007) Growth hormone IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society.17:392-8
  • Plasma vitamin C is inversely related to body mass index and waist circumference but not to plasma adiponectin in nonsmoking adults.
    Carol S Johnston, Bonnie L Beezhold, Bo Mostow, Pamela D Swan (2007) The Journal of nutrition.137:1757-62
  • Plasma adiponectin concentration in healthy pre- and postmenopausal women: relationship with body composition, bone mineral, and metabolic variables.
    Jaak Jürimäe, Toivo Jürimäe (2007) American journal of physiology. Endocrinology and metabolism.293:E42-7
  • Lack of association between serum adiponectin levels and the Pro12Ala polymorphism in Asian Indians.
    V Radha, K S Vimaleswaran, S Babu, R Deepa, M Anjana, S Ghosh, P P Majumder, M R S Rao, V Mohan (2007) Diabetic medicine : a journal of the British Diabetic Association.24:398-402
  • Serum adiponectin is related to plasma high-density lipoprotein cholesterol but not to plasma insulin-concentration in healthy children: the FLVS II study.
    Adrien Kettaneh, Barbara Heude, Jean-Michel Oppert, Philipp Scherer, David Meyre, Jean-Michel Borys, Pierre Ducimetiere, Marie-Aline Charles (2006) Metabolism: clinical and experimental.55:1171-6
  • Bone metabolism in elite male rowers: adaptation to volume-extended training.
    Jaak Jürimäe, Priit Purge, Toivo Jürimäe, Serge P von Duvillard (2006) European journal of applied physiology.97:127-32
  • Effect of rosiglitazone on endothelial function and inflammatory markers in patients with the metabolic syndrome.
    Katherine Esposito, Miryam Ciotola, Diego Carleo, Bruno Schisano, Franco Saccomanno, Ferdinando Carlo Sasso, Domenico Cozzolino, Roberta Assaloni, Domenico Merante, Antonio Ceriello, Dario Giugliano (2006) Diabetes care.29:1071-6
  • Adiponectin and stress hormone responses to maximal sculling after volume-extended training season in elite rowers.
    Jaak Jürimäe, Priit Purge, Toivo Jürimäe (2006) Metabolism: clinical and experimental.55:13-9
  • Maternal plasma adiponectin concentrations at 24 to 31 weeks of gestation: negative association with gestational diabetes mellitus.
    Po-Jung Tsai, Chun-Hsien Yu, Shih-Penn Hsu, Yu-Hsiang Lee, I-Tsan Huang, Su-Chen Ho, Chun-Hong Chu (2005) Nutrition (Burbank, Los Angeles County, Calif.).21:1095-9
  • Effects of the PPARgamma agonist pioglitazone on lipoprotein metabolism in patients with type 2 diabetes mellitus.
    Kazunori Nagashima, Carlos Lopez, Daniel Donovan, Colleen Ngai, Nelson Fontanez, André Bensadoun, Jamila Fruchart-Najib, Steve Holleran, Jeffrey S Cohn, Rajasekhar Ramakrishnan, Henry N Ginsberg (2005) The Journal of clinical investigation.115:1323-32
  • Association of low adiponectin levels with the metabolic syndrome--the Chennai Urban Rural Epidemiology Study (CURES-4).
    Viswanathan Mohan, Raj Deepa, Rajendra Pradeepa, Karani Santhanakrishnan Vimaleswaran, Anjana Mohan, Kaliaperunal Velmurugan, Venkatesan Radha (2005) Metabolism: clinical and experimental.54:476-81
  • Energy expenditure and adaptive responses to an acute hypercaloric fat load in humans with lipodystrophy.
    David B Savage, Peter R Murgatroyd, V Krishna Chatterjee, Stephen O'Rahilly (2005) The Journal of clinical endocrinology and metabolism.90:1446-52
  • Peripheral blood concentrations of adiponectin, an adipocyte-specific plasma protein, in normal pregnancy and preeclampsia.
    Katsuhiko Naruse, Mineo Yamasaki, Hideshi Umekage, Toshiyuki Sado, Yoshiharu Sakamoto, Hajime Morikawa (2005) Journal of reproductive immunology.65:65-75
  • Adiponectin is altered after maximal exercise in highly trained male rowers.
    Jaak Jürimäe, Priit Purge, Toivo Jürimäe (2005) European journal of applied physiology.93:502-5
  • Serum adiponectin concentrations in newborn infants in early postnatal life.
    Tomohiro Kamoda, Hisako Saitoh, Makoto Saito, Masatoshi Sugiura, Akira Matsui (2004) Pediatric research.56:690-3
  • Cord plasma concentrations of adiponectin and leptin in healthy term neonates: positive correlation with birthweight and neonatal adiposity.
    Po-Jung Tsai, Chun-Hsien Yu, Shih-Penn Hsu, Yu-Hsiang Lee, Chuen-Hua Chiou, Yu-Wen Hsu, Su-Chen Ho, Chun-Hong Chu (2004) Clinical endocrinology.61:88-93
  • Effect of liposuction on insulin resistance and vascular inflammatory markers in obese women.
    G Giugliano, G Nicoletti, E Grella, F Giugliano, K Esposito, N Scuderi, F D'Andrea (2004) British journal of plastic surgery.57:190-4
  • Ethnic differences in adiponectin levels.
    Matthew W Hulver, Ousama Saleh, Kenneth G MacDonald, Walter J Pories, Hisham A Barakat (2004) Metabolism: clinical and experimental.53:1-3
  • Insulin resistance, intramyocellular lipid content, and plasma adiponectin in patients with type 1 diabetes.
    Gianluca Perseghin, Guido Lattuada, Massimo Danna, Lucia Piceni Sereni, Paola Maffi, Francesco De Cobelli, Alberto Battezzati, Antonio Secchi, Alessandro Del Maschio, Livio Luzi (2003) American journal of physiology. Endocrinology and metabolism.285:E1174-81
  • Adiponectin is present in cord blood but is unrelated to birth weight.
    Robert S Lindsay, James D Walker, Peter J Havel, Barbara A Hamilton, Andrew A Calder, Frank D Johnstone (2003) Diabetes care.26:2244-9
  • Effect of weight loss and lifestyle changes on vascular inflammatory markers in obese women: a randomized trial.
    Katherine Esposito, Alessandro Pontillo, Carmen Di Palo, Giovanni Giugliano, Mariangela Masella, Raffaele Marfella, Dario Giugliano (2003) JAMA : the journal of the American Medical Association.289:1799-804
  • Adiponectin is not altered with exercise training despite enhanced insulin action.
    Matthew W Hulver, Donghai Zheng, Charles J Tanner, Joseph A Houmard, William E Kraus, Cris A Slentz, Madhur K Sinha, Walter J Pories, Kenneth G MacDonald, G Lynis Dohm (2002) American journal of physiology. Endocrinology and metabolism.283:E861-5

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    Somente para pesquisa, não é destinado ao uso terapêutico ou diagnóstico.

    Código do ProdutoHADP-61HK
    Tipo de Produto RIE Obesidade e Diabetes
    Fornecedor MILLIPORE
    Quantidade125
    EspécieHumano
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