Insulina Humana-Específica - RIE

Descrição: Human Insulin-Specific RIA

Informação: Insulina, um hormônio 5.8 kDa é secretado pelas células beta de ilhota no pâncreas. Este kit dosa especificamente insulina humana sem reatividade cruzada para pró-insulina intacta.

Aplicações: Radioimunoensaio

Incubação: Overnight, incubação de equilíbrio a temperatura ambiente.

Volume de Amostra: 100 µL soro, plasma, ou meio de cultura de tecido

Reatividade: Humano

Áreas Terapêuticas:

  • Obesidade
  • Desordens Metabólicas

Linearidade de Diluição: 86–108%

Precisão, %:

  • Inter-ensaio: 2.9–6.0
  • Intra-ensaio: 2.2–4.4

Exatidão:

93–100%

Desempenho:

  • ED80 = 7 ± 1 µU/ml
  • ED50 = 26 ± 3 µU/ml
  • ED20 = 102 ± 10 µU/ml

Intervalo de Curva Padrão: 2–200 µU/mL

Especificidade:

Human Insulin 100% Intact Human Proinsulin <0.2% Des 31, 32 HPI <0.2% Des 64, 65 HPI 76% Porcine Insulin 100% Porcine Proinsulin <3% Canine Insulin 100% Bovine Insulin 62% Bovine Prosinsulin <1% Rat Insulin <0.1% Glucagon Not Detectable Somatos

 

DOCUMENTO

Protocolo

REFERÊNCIA DE PUBLICAÇÕES PARA ESTE KIT

  • Misfolded proinsulin affects bystander proinsulin in neonatal diabetes.
    Hodish I, Liu M, Rajpal G, Larkin D, Holz RW, Adams A, Liu L, Arvan P (2010) The Journal of biological chemistry.285:685-94
  • Effects of glucose and insulin on acyl ghrelin and desacyl ghrelin, leptin, and adiponectin in pregnant women with diabetes.
    Gibson W, Liu J, Gaylinn B, Thorner MO, Meneilly GS, Babich SL, Thompson D, Chanoine JP (2009) Metabolism: clinical and experimental
  • Effect of intensity of resistance exercise on postprandial lipemia.
    Arpit Singhal, Jennifer L Trilk, Nathan T Jenkins, Kevin A Bigelman, Kirk J Cureton (2009) Journal of applied physiology (Bethesda, Md. : 1985).106:823-9
  • Metabolic and endocrine profiles in response to systemic infusion of fructose and glucose in rhesus macaques.
    Sean H Adams, Kimber L Stanhope, Ryan W Grant, Bethany P Cummings, Peter J Havel (2008) Endocrinology.149:3002-8
  • The muscle protein synthetic response to carbohydrate and protein ingestion is not impaired in men with longstanding type 2 diabetes.
    Ralph J Manders, René Koopman, Milou Beelen, Annemie P Gijsen, Will K Wodzig, Wim H Saris, Luc J van Loon (2008) The Journal of nutrition.138:1079-85
  • Association between KCNJ11 E23K genotype and cardiovascular and glucose metabolism phenotypes in older men and women.
    Yang Yi, Liu Dongmei, Dana A Phares, Edward P Weiss, Josef Brandauer, James M Hagberg (2008) Experimental physiology.93:95-103
  • Proinsulin maturation, misfolding, and proteotoxicity.
    Ming Liu, Israel Hodish, Christopher J Rhodes, Peter Arvan (2007) Proceedings of the National Academy of Sciences of the United States of America.104:15841-6
  • The effects of combined versus selective adrenergic blockade on left ventricular and systemic hemodynamics, myocardial substrate preference, and regional perfusion in conscious dogs with dilated cardiomyopathy.
    Lazaros A Nikolaidis, Indu Poornima, Pratik Parikh, Megan Magovern, You-Tang Shen, Richard P Shannon (2006) Journal of the American College of Cardiology.47:1871-81
  • Co-ingestion of a protein hydrolysate with or without additional leucine effectively reduces postprandial blood glucose excursions in Type 2 diabetic men.
    Ralph J Manders, René Koopman, Wendy E Sluijsmans, Robin van den Berg, Kees Verbeek, Wim H Saris, Anton J Wagenmakers, Luc J van Loon (2006) The Journal of nutrition.136:1294-9
  • C-reactive protein genotype affects exercise training-induced changes in insulin sensitivity.
    Thomas O Obisesan, Christiaan Leeuwenburgh, Robert E Ferrell, Dana A Phares, Jennifer A McKenzie, Steven J Prior, James M Hagberg (2006) Metabolism: clinical and experimental.55:453-60
  • Diabetes mellitus and serum carotenoids: findings of a population-based study in Queensland, Australia.
    Terry Coyne, Torukiri I Ibiebele, Peter D Baade, Annette Dobson, Christine McClintock, Sophie Dunn, Dympna Leonard, Jonathan Shaw (2005) The American journal of clinical nutrition.82:685-93
  • Influence of vitamin D receptor gene polymorphisms and 25-hydroxyvitamin D on blood pressure in apparently healthy subjects.
    Salomé Muray, Eva Parisi, Anna Cardús, Lourdes Craver, Elvira Fernández (2003) Journal of hypertension.21:2069-75
  • Engineering physiologically regulated insulin secretion in non-beta cells by expressing glucagon-like peptide 1 receptor.
    L Wu, W Nicholson, C-Y Wu, M Xu, A McGaha, M Shiota, A C Powers (2003) Gene therapy.10:1712-20
  • Use of sulfate production as a measure of short-term sulfur amino acid catabolism in humans.
    M J Hamadeh, L J Hoffer (2001) American journal of physiology. Endocrinology and metabolism.280:E857-66
  • Effect of feed restriction on adipose tissue transcript concentrations in genetically lean and obese pigs.
    R L McNeel, S T Ding, E O Smith, H J Mersmann (2000) Journal of animal science.78:934-42

    • Somente para pesquisa, não é destinado ao uso terapêutico ou diagnóstico

    Código do ProdutoHI-14K
    Tipo de Produto RIE Obesidade e Diabetes Insulina
    Fornecedor MILLIPORE
    Quantidade250 testes
    EspécieHumano
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