Adiponectina de Camundongo RIE

Mouse Adiponectin RIA

Adiponectina é identificada como uma adipocina de 30 kDa que é secretada no tecido adiposo e influência o metabolismo sistêmico.

Aplicações: Radioimunoensaio

Notas: 
Incubação overnight a temperatura ambiente.
Volume de amostra - <2 µL de soro ou plasma, ou <100 µL de meio de cultura de tecido

Reatividade: Camundongo

Precisão, %:

• Inter-ensaio: 6.6–8.2
• Intra-ensaio: 3.7–4.4

Sensibilidade: 0.78 ng/mL

Áreas terapêuticas:

Exatidão: 96–117%

Intervalo da curva: 0.78–100 ng/mL

Especificidade:

Adiponectina humana - 20%

Adiponectina de Rato - Bastante alta *

UniProt Number:

• Q15848
• Q60994

Desempenho:

• ED₈₀ = 1.2 ± 0.15 ng/mL
• ED₅₀ = 4.5 ± 0.69 ng/mL
• ED₂₀ = 17.6 ± 3.3 ng/mL

Proteína: Adiponectina

Somente para pesquisa, não é destinado ao uso terapêutico ou diagnóstico.

Referências

High-salt diet increases plasma adiponectin levels independent of blood pressure in hypertensive rats: the role of the renin-angiotensin-aldosterone system.
Kamari Y, Shimoni N, Koren F, Peleg E, Sharabi Y, Grossman E (2010) Journal of hypertension.28:95-101

Distinct dysregulation of lipid metabolism by unliganded thyroid hormone receptor isoforms.
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Dietary L-arginine supplementation reduces white fat gain and enhances skeletal muscle and brown fat masses in diet-induced obese rats.
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Partial leptin deficiency favors diet-induced obesity and related metabolic disorders in mice.
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Corticosterone inhibits the lipid-mobilizing effects of oleoyl-estrone in adrenalectomized rats.
Maria del Mar Grasa, Marta Serrano, José Antonio Fernández-López, Marià Alemany (2007) Endocrinology.148:4056-63

The conjugated linoleic acid ester of estrone induces the mobilisation of fat in male Wistar rats.
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Impaired adipogenesis caused by a mutated thyroid hormone alpha1 receptor.
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Improvement of insulin sensitivity after peroxisome proliferator-activated receptor-alpha agonist treatment is accompanied by paradoxical increase of circulating resistin levels.
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Limited therapeutic efficacy of pioglitazone on progression of hepatic fibrosis in rats.
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Leptin improves insulin resistance and hyperglycemia in a mouse model of type 2 diabetes.
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Consuming fructose-sweetened beverages increases body adiposity in mice.
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Genetic background (C57BL/6J versus FVB/N) strongly influences the severity of diabetes and insulin resistance in ob/ob mice.
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Insulin resistance in the liver-specific IGF-1 gene-deleted mouse is abrogated by deletion of the acid-labile subunit of the IGF-binding protein-3 complex: relative roles of growth hormone and IGF-1 in insulin resistance.
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Liver peroxisome proliferator-activated receptor gamma contributes to hepatic steatosis, triglyceride clearance, and regulation of body fat mass.
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Transplantation of adipose tissue lacking leptin is unable to reverse the metabolic abnormalities associated with lipoatrophy.
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