Leptina Humana Sensível - RIE

Sensitive Human Leptin RIA

Leptina é sintetizada nos adipócitos como uma molécula de 16kD e seus níveis circulantes é diretamente proporcional a quantidade total de gordura corpórea.  

Aplicações: 

Ensaio de equilíbrio overnight, incubação a temperatura ambiente. Volume de Amostra < 100 µL, soro, plasma ou meio de cultura de tecido.

Reatividade: Humano

Precisão, %:

• Inter-ensaio: 3.2–8.9
• Intra-ensaio: 3.7–7.5 

Áreas terapêuticas:

• Diabetes
• Obesidade
• Desordem Metabólica

Exatidão: 104–118%

Intervalo da curva: 0.05–10 ng/mL

Reatividade Cruzada:
Leptina Humana 100% / Leptina de Camundongo <2% / Insulina Humana - Não Detectável / Glucagon- Não Detectável / Leptina de rato <1% / SRIF- Não Detectável / Leptina Canina <0.2%

Gene Symbol:

• LEP
• leptin
• OB
• obesity
• OBS

UniProt Number: P41159

Entrez Gene Number: NM_000230.1

Linearidade de diluição: 72–104%

Desempenho:

• ED₈₀ = 0.28 ± 0.04 ng/ml
• ED₅₀ = 1.05 ± 0.12 ng/ml
• ED₂₀ = 4.48 ± 0.66 ng/ml

Proteína: Leptina (Obese Protein, OB)

DOCUMENTO

Protocolo

REFERÊNCIA DE PUBLICAÇÕES PARA ESTE KIT

Impact of acute sleep restriction on cortisol and leptin levels in young women.

Abstract

Sleep restriction alters hormone patterns and appetite in men, but less is known about effects on women. We assessed effects of overnight sleep restriction on cortisol and leptin levels and on appetite in young women. Participants' baseline sleep duration and eating habits were monitored for a week before the study. Salivary cortisol and leptin were sampled from fifteen healthy women (aged 18-25) during two consecutive days: first after a 10h overnight sleep opportunity (Baseline day) and then after a night including only 3h sleep (Post sleep-restriction day). Participants also completed appetite questionnaires on both days. Sleep restriction significantly reduced morning cortisol levels (p=0.02), elevated morning leptin levels (p=0.04), elevated afternoon/evening cortisol area under the curve values (p=0.008), and slowed the decline in cortisol concentration during the day (p=0.04). Hunger and craving scores did not differ significantly between days. A single night of restricted sleep affected cortisol rhythms and morning leptin levels in young women. (c) 2010 Elsevier Inc. All rights reserved.

Reference Links

Body mass index and serum leptin concentration independently estimate percentage body fat in older adults.

Abstract

BACKGROUND: Because serum concentrations of leptin, a hormone produced by adipocytes, can be relatively reliably and inexpensively measured, it may be considered complementary to, or even a substitute for, body mass index (BMI) as a measure of adiposity. OBJECTIVE: We examined the ability of BMI and leptin concentrations, separately and together, to estimate total percentage fat in older adults. DESIGN: Total percentage fat measured by dual-energy X-ray absorptiometry and fasting serum leptin concentrations were measured in 2911 well-functioning 70-79-y-old participants (42% black, 51% women) in the Health, Aging, and Body Composition Study. RESULTS: Mean (+/-SD) total percentage fat was 29.2 +/-5.0% in men and 40.5 +/- 5.7% in women, and the geometric mean (+/-SD) serum leptin concentration was 5.6 +/- 2.5 ng/mL in the men and 16.4 +/- 2.3 ng/mL in the women. Among men, total percentage fat was strongly associated with both BMI (R(2) = 0.56) and leptin (R(2) = 0.57) in separate linear regression analyses and in a combined linear regression analysis (R(2) = 0.68). Similarly, among women, total percentage fat was associated with both BMI (R(2) = 0.65) and leptin (R(2) = 0.54) separately and in combination (model R(2) = 0.71). Independent relations of BMI and leptin with total percentage fat were also found among both black and white participants. With the population divided into quintiles according to percentage fat, BMI and serum leptin correctly classified 49% of men and 50% of women in the correct quintile. CONCLUSIONS: Among older adults, total percentage fat was better estimated by using both serum leptin concentrations and BMI than by using either alone. However, their performance does not suggest that they can substitute for more accurate measures.

Somente para pesquisa, não é destinado ao uso terapêutico ou diagnóstico.